Cutting mesh structures in Blender

Introduction

3D rendering of a mouse pancreatic β cell showing the complete nucleus (white), all microtubules (red), and cut-outs of insulin secretory granules (light orange), the mitochondria (blue), the Golgi apparatus (green), and the plasma membrane (transparent).

The dataset shown in this tutorial originates from the same publication.


Preparation

Converting volumetric data into meshes

Separate tutorial - click this box!

Converting Volumes to Meshes with VTK

This tutorial will guide you through converting 3D pixel datasets, such as labelmaps and masks, into 3D meshes using VTK.

Preparation

Creating a Blender scene from meshes

Separate tutorial - click this box!

Rendering in Blender

In this section, we will cover specific Blender features to help you create beautiful renderings from scientific datasets. Blender is a powerful tool for this purpose, but it has a steep learning curve, so we’ll break it down step-by-step.

Intro to modifiers in Blender


Add shape which will be removed from the dataset

Steps:

  1. Add a cube, sphere, or any shape that represents the area you want to remove from the dataset.
  2. Position the object over the part of the mesh you want to cut.

Use boolean modifier with shape

Boolean Modifier: Used for cutting, union, or intersection operations between meshes.

Steps:

  1. Select your mesh object.
  2. In the Modifiers tab, add a Boolean modifier.
  3. Set the operation to “Difference” and select the cutting object.
  4. Apply the modifier to perform the cut permanently.

Hide shape and render

Final Steps:

  1. Hide or delete the cutting shape (if you applied the modifier).
  2. Render the scene to reveal the internal structures of the mesh.